Linked Life Data

6179257

Source:http://linkedlifedata.com/resource/pubmed/id/6179257

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1982-9-24
pubmed:abstractText
Clones of alloreactive T-lymphocytes were isolated from 6-day-old mixed leukocyte cultures (MLCs) by limiting dilution in the presence of filler cells (either pooled irradiated peripheral blood mononuclear cells (PBMCx) or autologous PBMCx) followed by expansion of clonal progeny with Interleukin 2 (IL 2). Using allogeneic IL 2, produced by stimulating pooled PBMC with phytohaemagglutinin (PHA), in conjunction with pooled filler cells for the limiting dilution cloning, over half the clones obtained had primed lymphocyte typing (PLT) function. The majority of these discriminated priming cell HLA-D type with fine specificity. However, clones which were restimulated by cells of specificities other than the priming HLA-D type were also found, and, moreover, some ("wild" clones) were not restimulated by the original priming cells. Antigens responsible for restimulating these clones included hitherto undetected HLA-encoded products (different from HLA-A, B, C, D, DR, MT, MB or SB). Such "wild" clones were also obtained after cloning procedures in which autologous filler cells were used. Autologous IL 2, depleted of mitogenic PHA, and used in conjunction with autologous filler cells, also failed to prevent generation of such clones. These results indicate that although the PLT-functional lymphocyte population alloactivated by a single HLA-DR/Dw specificity expressed by HLA-D homozygous stimulating cells consists of a majority of clones exclusively recognising specific priming-type HLA-DR/Dw products, it also contains clones recognising different HLA-DR/Dw specificities, as well as clones recognising lymphocyte stimulating determinants hitherto undetected by serological or cellular methods.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0001-2815
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
269-88
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Discrete PLT clones detecting HLA-D and new HLA-non-D encoded cell surface determinants.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't