Linked Life Data

6176223

Source:http://linkedlifedata.com/resource/pubmed/id/6176223

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1982-6-21
pubmed:abstractText
Human blood lymphocytes were coated with increasing amounts of human kappa chain (2-85mug/10(7) cells) through the linking reagent CrCl(3). These cells were then exposed to small unilamellar liposomes composed of egg phosphatidylcholine, cholesterol and phosphatidic acid (molar proportions 7:7:1) containing carboxyfluorescein and/or (111)In-labelled bleomycin and bearing (131)I-labelled affinity chromatography-purified or non-purified anti-(kappa-chain) immunoglobulin G (IgG) [see the preceding paper, Gregoriadis, Meehan & Mah (1981) Biochem. J.200, 203-210]. In some experiments liposomes contained [(14)C]phosphatidylcholine. (1) Lymphocytes (10(7)) coated with 2-85mug of kappa chain and exposed to liposomes devoid of IgG or bearing non-purified anti-(kappa chain) IgG bound only a small proportion of the liposomal markers. Even with liposomes bearing the purified anti-(kappa chain) IgG, uptake of the labels improved only slightly for cells coated with up to 10mug of kappa chain. However, with higher concentrations of the antigen on the cell surface, binding was improved considerably to reach values of 31% ((111)In-labelled bleomycin) and 43% ((131)I-labelled IgG) of added liposomes for cells coated with 85mug of kappa chain. (2) Lymphocytes coated with kappa chain were exposed to liposomes bearing increasing amounts (0-180mug/0.9mg of egg phosphatidylcholine) of purified anti-(kappa chain) IgG. It was found that under the present conditions, binding of all three markers ((111)In-labelled bleomycin, (131)I-labelled IgG and [(14)C]phosphatidylcholine) was directly proportional to the concentration of IgG on the liposomal surface. However, uptake values remained unchanged above 90mug of IgG. (3) Antibody-mediated uptake of liposomes by cells coated with the corresponding antigen without loss of their metabolic activities may provide a method of efficient targeting.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6176223-352245, http://linkedlifedata.com/resource/pubmed/commentcorrection/6176223-4434987, http://linkedlifedata.com/resource/pubmed/commentcorrection/6176223-50067, http://linkedlifedata.com/resource/pubmed/commentcorrection/6176223-5305881, http://linkedlifedata.com/resource/pubmed/commentcorrection/6176223-6114292, http://linkedlifedata.com/resource/pubmed/commentcorrection/6176223-6803776, http://linkedlifedata.com/resource/pubmed/commentcorrection/6176223-7001454, http://linkedlifedata.com/resource/pubmed/commentcorrection/6176223-70733, http://linkedlifedata.com/resource/pubmed/commentcorrection/6176223-7207015, http://linkedlifedata.com/resource/pubmed/commentcorrection/6176223-7378067, http://linkedlifedata.com/resource/pubmed/commentcorrection/6176223-7410345, http://linkedlifedata.com/resource/pubmed/commentcorrection/6176223-7423203, http://linkedlifedata.com/resource/pubmed/commentcorrection/6176223-7428926, http://linkedlifedata.com/resource/pubmed/commentcorrection/6176223-7442804
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
200
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
211-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Interaction of antibody-bearing small unilamellar liposomes with antigen-coated cells. The effect of antibody and antigen concentration on the liposomal and cell surface respectively.
pubmed:publicationType
Journal Article, In Vitro