| pubmed-article:6174829 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:6174829 | lifeskim:mentions | umls-concept:C1882814 | lld:lifeskim |
| pubmed-article:6174829 | lifeskim:mentions | umls-concept:C0002712 | lld:lifeskim |
| pubmed-article:6174829 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:6174829 | lifeskim:mentions | umls-concept:C0185125 | lld:lifeskim |
| pubmed-article:6174829 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:6174829 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:6174829 | lifeskim:mentions | umls-concept:C0303920 | lld:lifeskim |
| pubmed-article:6174829 | lifeskim:mentions | umls-concept:C1710236 | lld:lifeskim |
| pubmed-article:6174829 | lifeskim:mentions | umls-concept:C0205349 | lld:lifeskim |
| pubmed-article:6174829 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:6174829 | pubmed:dateCreated | 1982-5-27 | lld:pubmed |
| pubmed-article:6174829 | pubmed:abstractText | A modified fluorescence assay for alpha-amylase activity is described. The method employs amylopectin anthranilate as substrate and offers the advantages of economy of time and resources over a previously described technique using the same substrate. The sample containing alpha-amylase is incubated with the substrate for 5 min at 30 degree C in a final volume of 750 microliter. The fluorescent products of the reaction are separated from the substrate by the addition of methanol, and the methanol-soluble fluorescence is measured in a fluorescence spectrometer. A highly reproducible linear relationship between fluorescence and alpha-amylase activity is obtained for enzyme activities up to 2 units. The absolute sensitivity of the assay under these conditions was estimated to be 0.02 EU (= 0.08 EU ml-1). The application of the assay method to a study of the effects of isoprenaline and substance P-like peptides on the release of alpha-amylase from rat parotid gland slices is described. The assay is particularly suitable for studies on agonists, such as substance P, which have a low ceiling effect in terms of amylase release. | lld:pubmed |
| pubmed-article:6174829 | pubmed:language | eng | lld:pubmed |
| pubmed-article:6174829 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6174829 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:6174829 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6174829 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6174829 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6174829 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6174829 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:6174829 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:6174829 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:6174829 | pubmed:issn | 0160-5402 | lld:pubmed |
| pubmed-article:6174829 | pubmed:author | pubmed-author:JordanC CCC | lld:pubmed |
| pubmed-article:6174829 | pubmed:author | pubmed-author:OwenD GDG | lld:pubmed |
| pubmed-article:6174829 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:6174829 | pubmed:volume | 6 | lld:pubmed |
| pubmed-article:6174829 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:6174829 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:6174829 | pubmed:pagination | 281-93 | lld:pubmed |
| pubmed-article:6174829 | pubmed:dateRevised | 2009-11-3 | lld:pubmed |
| pubmed-article:6174829 | pubmed:meshHeading | pubmed-meshheading:6174829-... | lld:pubmed |
| pubmed-article:6174829 | pubmed:meshHeading | pubmed-meshheading:6174829-... | lld:pubmed |
| pubmed-article:6174829 | pubmed:meshHeading | pubmed-meshheading:6174829-... | lld:pubmed |
| pubmed-article:6174829 | pubmed:meshHeading | pubmed-meshheading:6174829-... | lld:pubmed |
| pubmed-article:6174829 | pubmed:meshHeading | pubmed-meshheading:6174829-... | lld:pubmed |
| pubmed-article:6174829 | pubmed:meshHeading | pubmed-meshheading:6174829-... | lld:pubmed |
| pubmed-article:6174829 | pubmed:meshHeading | pubmed-meshheading:6174829-... | lld:pubmed |
| pubmed-article:6174829 | pubmed:meshHeading | pubmed-meshheading:6174829-... | lld:pubmed |
| pubmed-article:6174829 | pubmed:meshHeading | pubmed-meshheading:6174829-... | lld:pubmed |
| pubmed-article:6174829 | pubmed:meshHeading | pubmed-meshheading:6174829-... | lld:pubmed |
| pubmed-article:6174829 | pubmed:year | 1981 | lld:pubmed |
| pubmed-article:6174829 | pubmed:articleTitle | A modified amylase assay, using a fluorescent substrate, and its application to a study of the rat parotid gland in vitro. | lld:pubmed |
| pubmed-article:6174829 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:6174829 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:6174829 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:6174829 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
