Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1982-5-27
pubmed:abstractText
Parameters relating to RNA synthesis were measured after a temperature shift from 30 to 42 degrees C, in a relA+ and relA- isogenic pair of Escherichia coli strains containing a temperature-sensitive valyl tRNA synthetase. The following results were obtained: (i) the rRNA chain growth rate increased 2-fold in both strains; (ii) newly synthesized rRNA became unstable in both strains; (iii) the stable RNA gene activity (rRNA and tRNA, measured as stable RNA synthesis rate relative to the total instantaneous rate of RNA synthesis) decreased 1.7-fold in the relA+ strain and increased 1.9-fold in the relA mutant; and (iv) the RNA polymerase activity (measured by the percentage of total RNA polymerase enzyme active in transcription an any instant) decreased from 20 to 3.6% in the relA+ strain and remained unchanged (or increased at most to 22%) in the relA mutant. It is suggested that both rRNA gene activity and the RNA polymerase activity depend on the intracellular concentration of guanosine tetraphosphate, whereas the altered chain elongation rate and stability of rRNA are temperature or amino acid starvation effects, respectively, without involvement of relA function.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-1097698, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-1108019, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-13985165, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-324974, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-324975, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-338910, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-351406, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-4197337, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-4562313, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-4576025, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-4576590, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-4581288, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-4850204, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-4877268, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-4885263, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-4897796, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-4901708, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-4935355, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-4941474, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-5331245, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-5336166, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-6154044, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-6159345, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-6159476, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-787760, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-789334, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-8211, http://linkedlifedata.com/resource/pubmed/commentcorrection/6174501-94252
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
150
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
168-79
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
relA-dependent RNA polymerase activity in Escherichia coli.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.