Linked Life Data

6173092

Source:http://linkedlifedata.com/resource/pubmed/id/6173092

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1982-4-12
pubmed:abstractText
Brain primary cultures from newborn rat hemispheres have been further characterized. THe cultures contained the glial fibrillary acidic protein (GFAp; alpha-albumin), as analyzed quantitatively. GFAp (alpha-albumin) increased in concentration during growth of cultures and during maturation of the intact rat brain. FITC-labelled antibodies against GFAp (alpha-albumin) reacted specifically with astrocyte-like cells. Theses cells were also positive after Rio-Hortega's silver carbonate staining for astrocytes. No myelin basic protein (MBP) was detected in cultures, neither by quantitative measurements nor by immunofluorescent histochemistry. Negative results were also obtained for the myelin specific enzyme 2', 3'-cyclic nucleotide 3'-phosphohydrolase (CNP), e. e. the enzyme was at the same level in the cultures of different ages as in 3-day-old rat hemispheres. However, in rat brain increasing concentrations of MBP and CNP were found during maturation. This means that no myelin and thereby probably no or few differentiated oligodendroglial cells were present in the cultures, where the predominate cell type was of astroglial nature.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
231
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
173-83
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Brain primary culture-- a characterization (part II).
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't