Linked Life Data

6172581

Source:http://linkedlifedata.com/resource/pubmed/id/6172581

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1982-3-22
pubmed:abstractText
1. Plasma proteins have been demonstrated to be present in early fetal sheep brain in amounts which cannot be accounted for by blood contamination. 2. The distribution of alpha-fetoprotein, albumin, fetuin, alpha 1-antitrypsin and transferrin has been studied by immunoassay of extracts from brain homogenates and by immunoperoxidase histochemistry of fetal brains between 37 and 125 days gestation (term is 150 days). 3. At 35 days gestation fetuin and albumin were quantitatively the most important proteins in fetal brain, both as estimated by extraction and by immunohistochemistry. Both of these proteins, and also alpha-fetoprotein and alpha 1-antitrypsin, declined considerably in amount by 60 days gestation. After 60 days the concentrations of albumin and alpha-fetoprotein were not significantly different from that due to blood contamination and only occasional cells could be demonstrated by immunohistochemistry. Fetuin and alpha 1-antitrypsin were present in reduced but significant amounts at least up to 125 days gestation. 4. The immunohistochemical results showed that considerable numbers of immature neurones were stained for some plasma proteins. Fetuin positive cells predominated both in terms of the larger number of cells which stained at 35-40 days gestation and in the persistence of positive cells up to 125 days gestation. Numerous cells in the neuroependymal layer and in several layers of the developing cortical plate were positive, especially early in gestation. Only a few transferrin or alpha 1-antitrypsin positive cells were observed. 5. Permeability of the blood-brain barrier to sheep or human serum albumin (labelled with 125I) was tested in 60 day fetal sheep by intravenous injection and estimation of brain radioactivity at 3 or 6 hr with allowance for blood contamination. Only a very small (but significant) penetration of protein was detected. Unlike penetration of protein into c.s.f. at the same age, it did not reach its natural steady state in brain. 6. It is concluded that the blood-brain barrier to protein is well developed in the immature fetal sheep but that developing neurones probably acquire certain plasma proteins directly from the c.s.f. when they are differentiating in the neuroependyma. The subsequent distribution of plasma protein positive cells in different brain regions is suggested to be due to the migration of developing neurones for the neuroependyma although the possibility of local synthesis of plasma proteins has not been excluded.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172581-1274618, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172581-14097352, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172581-22861, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172581-4114448, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172581-4180949, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172581-4203033, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172581-4601383, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172581-490348, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172581-4956917, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172581-5707962, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172581-5765759, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172581-6155467, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172581-6155468, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172581-6157991, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172581-69448, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172581-75046, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172581-77294, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172581-82367, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172581-93719, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172581-95002
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-3751
pubmed:author
pubmed:issnType
Print
pubmed:volume
318
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
239-50
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Plasma proteins in fetal sheep brain: blood-brain barrier and intracerebral distribution.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't