Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1982-2-12
pubmed:abstractText
It is shown that non-proteolytic proteins can become covalently linked to alpha 2M (alpha 2-macroglobulin) during its reaction with proteinases, and that this probably occurs by the mechanism that leads to the covalent linking of proteinases described previously [Salvesen & Barrett (1980) Biochem. J. 187, 695-701]. The covalent linking of trypsin was at least partly dependent on the presence of unblocked lysine side chains on the protein. The covalent linking of proteinases was inhibited by nucleophiles of low Mr, and these compounds were themselves linked to alpha 2M in a molar ratio approaching one per quarter subunit. Peptide "mapping" indicated that the site of proteinase-mediated incorporation of the amines was the same as that at which methylamine is incorporated in the absence of a proteinase. The nucleophile-reactive site revealed in alpha 2M after reaction with a proteinase was shown to decay with a t1/2 of 112 s, at pH 7.5. After the reaction with a proteinase or with methylamine, a free thiol group was detectable on each subunit of alpha 2M. We propose that the site for incorporation of methylamine in each subunit is a thiol ester, which in S-alpha 2M (the electrophoretically "slow" form) is sterically shielded from reaction with large nucleophiles, but is revealed as a highly reactive group, free from steric hindrance, after the proteolytic cleavage. We have designated the activated species of the molecule "alpha 2M".
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-12325371, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-13093634, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-320200, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-38283, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-4201304, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-474960, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-5415108, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-543547, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-5498633, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-58667, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-6154704, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-6156166, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-6161608, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-6204637, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-637870, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-6906229, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-6934510, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-73346, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-851219, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-85262, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-89116, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-91367, http://linkedlifedata.com/resource/pubmed/commentcorrection/6172116-92026
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
195
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
453-61
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Further characterization of the covalent linking reaction of alpha 2-macroglobulin.
pubmed:publicationType
Journal Article