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rdf:type |
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lifeskim:mentions |
umls-concept:C0005149,
umls-concept:C0006556,
umls-concept:C0020202,
umls-concept:C0028953,
umls-concept:C0086418,
umls-concept:C0162326,
umls-concept:C0182400,
umls-concept:C0204727,
umls-concept:C0205409,
umls-concept:C1522642,
umls-concept:C1524063,
umls-concept:C1883254
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pubmed:issue |
11
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pubmed:dateCreated |
1982-2-12
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pubmed:databankReference |
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pubmed:abstractText |
We have synthesized two sets of 15-base-long oligodeoxyribonucleotides corresponding to all possible coding sequences for a small portion of human beta 2-microglobulin. Labeled oligonucleotides were used as hybridization probes to screen bacterial clones containing cDNA sequences primed with oligo(dT) and inserted into the plasmid vector pBR322. One beta 2-microglobulin cDNA clone was detected in the 535 bacterial plasmid clones that were screened. The clone has been characterized by blotting and nucleotide sequence analysis. The cloned beta 2-microglobulin sequence contains 217 base pairs of the 3' untranslated region of the mRNA and 328 base pairs (97%) of the coding region.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-1144058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-118435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-158748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-210956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-232215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-301042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-35231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-360074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-372548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-388427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-4129801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-4175239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-4288580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-4517941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-4586824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-4747634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-4792294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-4868544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-5086539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-6159538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-6159584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-6159597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-6165997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-6166005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-6246363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-6997493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-7232206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-7465417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-822353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-87220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-88048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-91168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6171820-91170
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6613-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6171820-Amino Acid Sequence,
pubmed-meshheading:6171820-Base Sequence,
pubmed-meshheading:6171820-Beta-Globulins,
pubmed-meshheading:6171820-Cloning, Molecular,
pubmed-meshheading:6171820-DNA, Recombinant,
pubmed-meshheading:6171820-Escherichia coli,
pubmed-meshheading:6171820-Humans,
pubmed-meshheading:6171820-Nucleic Acid Hybridization,
pubmed-meshheading:6171820-Oligodeoxyribonucleotides,
pubmed-meshheading:6171820-Oligonucleotides,
pubmed-meshheading:6171820-Plasmids,
pubmed-meshheading:6171820-beta 2-Microglobulin
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pubmed:year |
1981
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pubmed:articleTitle |
Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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