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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1982-1-9
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pubmed:abstractText |
The pantothenic acid content of gramicidin S synthetase 2(GS 2) was estimated microbiologically with enzymes obtained from the wild strain and gramicidin S-lacking mutant strains of Bacillus brevis. Four mutant enzymes from BI-4, C-3, E-1, and E-2 lacked pantothenic acid. Other mutant enzymes from BII-3, BI-3, BI-9, and BI-2 contained the same amount of pantothenic acid as the wild-type enzyme. Pantothenic acid-lacking GS 2 belonged to group V of mutant enzymes, which could activate all amino acids related to gramicidin S; their complementary enzyme, gramicidin S synthetase 1(GS 1), lacked racemizing activity. To ascertain whether 4'-phosphopantetheine is involved in the formation of D-phenylalanyl-L-prolyl diketopiperazine (DKP) and gramicidin S, combinations were tested of intact GS 1 from the wild strain with various mutant GS 2 either containing or lacking pantothenic acid. Only the combinations of wild-type GS 1 with mutant GS 2 containing pantothenic acid could synthesize DKP. Combinations with pantothenic acid-lacking GS 2 also failed to elongate peptide chains. Pantothenic acid-lacking GS 2 could bind the four amino acids which constitute gramicidin S as acyladenylates and thioesters, but the binding abilities were lower than those of the wild-type enzyme and other mutant enzymes containing the pantothenic group.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Isomerases,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Gramicidin,
http://linkedlifedata.com/resource/pubmed/chemical/Multienzyme Complexes,
http://linkedlifedata.com/resource/pubmed/chemical/Pantothenic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Synthases,
http://linkedlifedata.com/resource/pubmed/chemical/Phenylalanine,
http://linkedlifedata.com/resource/pubmed/chemical/Piperazines,
http://linkedlifedata.com/resource/pubmed/chemical/gramicidin-S-synthetase 2
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-924X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
439-47
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pubmed:dateRevised |
2007-12-19
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pubmed:meshHeading |
pubmed-meshheading:6170631-Amino Acid Isomerases,
pubmed-meshheading:6170631-Amino Acids,
pubmed-meshheading:6170631-Bacillus,
pubmed-meshheading:6170631-Biological Assay,
pubmed-meshheading:6170631-Gramicidin,
pubmed-meshheading:6170631-Lactobacillus,
pubmed-meshheading:6170631-Multienzyme Complexes,
pubmed-meshheading:6170631-Mutation,
pubmed-meshheading:6170631-Pantothenic Acid,
pubmed-meshheading:6170631-Peptide Chain Elongation, Translational,
pubmed-meshheading:6170631-Peptide Synthases,
pubmed-meshheading:6170631-Phenylalanine,
pubmed-meshheading:6170631-Piperazines
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pubmed:year |
1981
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pubmed:articleTitle |
Absence of pantothenic acid in gramicidin S synthetase 2 obtained from some mutants of Bacillus brevis.
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pubmed:publicationType |
Journal Article
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