Linked Life Data

6168733

Source:http://linkedlifedata.com/resource/pubmed/id/6168733

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1981-11-22
pubmed:abstractText
The effect of trypan blue on the rates of DNA, RNA, and protein accumulation in rat embryos was investigated using a system of whole embryo culture. Embryos were isolated on the 10th gestational day (sperm day = Day 0) either 4 or 24 hr after maternal injection. The cultivation period was for 24 or 42 hr. The rate of DNA and RNA accumulation in the embryo following either a 4- or 24-hr previous maternal treatment was significantly decreased at at both 24 and 42 hr of cultivation. Protein values were significantly reduced after a 4-hr maternal exposure, but only slightly reduced after a 24-hr maternal exposure. No significant differences in DNA, RNA, or protein content were observed at the beginning of cultivation, following either a 4- or 24-hr previous maternal treatment. The percentage of embryos establishing a visceral yolk sac circulation was greatly reduced, and this supports the conclusion that trypan blue interferes with yolk sac function. The most obvious teratogenic insults include failure of neuropore closure, retention of a dorsiflexed position, and cranial edema.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-104X
pubmed:author
pubmed:issnType
Print
pubmed:volume
216
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
415-22
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Accumulation of DNA, RNA, and protein by cultured rat embryos following maternal administration of a teratogenic dose of trypan blue.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.