Linked Life Data

6167351

Source:http://linkedlifedata.com/resource/pubmed/id/6167351

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9 Pt 1
pubmed:dateCreated
1981-10-25
pubmed:abstractText
When two lines of gestational human choriocarcinoma cells (GCH-1 and GCH-2) were incubated with [35S]methionine, the labeled beta 2-microglobulin was not detected on sodium dodecyl sulfate:polyacrylamide gel electrophoresis after purification of the labeled protein with affinity chromatography, using rabbit antibody against beta 2-microglobulin as the ligand. In contrast, in the case of nongestational choriocarcinoma cells originating from male stomach cells, the incorporation of [35S]methionine into beta 2-microglobulin was observed. When polyadenylate-containing messenger RNA prepared from GCH-1 cells was incubated with [35S]methionine in a rabbit reticulocyte lysate system, the labeling of beta 2-microglobulin was not shown. On the other hand, when polyadenylate-containing messenger RNA from nongestational choriocarcinoma cells was incubated in the same cell-free system, labeled beta 2-microglobulin was detected. These results indicate that beta 2-microglobulin is not synthesized in gestational human choriocarcinoma cells and at least in the case of GCH-1, beta 2-microglobulin is not synthesized owing to the lack of effective messenger RNA for beta 2-microglobulin.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3639-41
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Lack of effective messenger RNA for beta 2-microglobulin in a gestational human choriocarcinoma cell line (GCH-1).
pubmed:publicationType
Journal Article, Comparative Study