Linked Life Data

6166861

Source:http://linkedlifedata.com/resource/pubmed/id/6166861

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1981-9-25
pubmed:abstractText
RNA from oocysts of Eimeria tenella was purified into poly(A)--RNA and poly(A)+-RNA fractions with affinity chromatography on oligo(dT)-cellulose. Gel electrophoresis, under denaturing conditions of the poly(A)--RNA fraction revealed two major RNAs with molecular weights of 1.27 and 0.65 X 10(6). The values for these components not only represent the ribosomal RNAs of this species; but they also resemble the molecular sizes of the non-mRNAs of many other lower eukaryotes. In vitro translation assays with a cell-free wheat germ system showed that the level of translatable mRNA in the poly(A)--RNA fraction was negligible. Virtually all the translatable mRNA in E. tenella was polyadenylated, that is, it contained poly(A)-tracts with more than 15 adenylate residues. Consequently some of the key components of the protein translational machinery of this organism are eukaryotic in nature.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0166-6851
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
133-42
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Ribosomal and translatable messenger RNA of Eimeria tenella.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't