Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-9-15
pubmed:abstractText
Retrograde transport of HRP and the fluorescent dye DAPI were used to double label spinothalamic tract cells with dual projections to thalamus and cerebellum. Cells containing both retrograde tracers were found in the intermediate gray zone and in the lateral spinal nucleus of the rat lumbosacral spinal cord. Double labeled cells were easily identified by the presence of granular HRP reaction, product and the blue fluorescence of DAPI. The use of HRP and DAPI provides an effective double labeling technique for the localization of neurons with nonoverlapping projections in the central and/or peripheral nervous system.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0165-0270
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
53-62
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
A retrograde double label tracing technique using horseradish peroxidase and the fluorescent dye 4'-,6-diamidino-2-phenylindole 2HC1 (DAPI).
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.