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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1981-9-15
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pubmed:abstractText |
We developed a method for production of antigen-specific, H-2-restricted T cell hybrids. The tumor cell partner in the fusions was itself a T cell hybrid, FS6-14.13.AG2 (or its derivatives), which could be induced to produce the growth factor, interleukin-2 (IL-2), in response to a challenge with concanavalin A, but had no known antigen specificity. The normal T cell partner in the fusions was a population of lymph node T cell blasts that had been highly enriched in antigen-specific, H-2-restricted T cells by in vivo immunization, followed by in vitro challenge with antigen and clonal expansion in IL-2-containing medium. These fusions produced hybrids that grew constitutively in culture. A sizable proportion of the hybrids demonstrated the ability to produce IL-2 in response to a challenge with specific antigen presented by irradiated spleen cells of the appropriate H-2 type. Four cloned antigen/H-2-specific hybrid lines were produced. AO-40.10 responded to chicken ovalbumin (OVA) when presented by I-A(k)-bearing cells. DC1.18.3 responded to the apo form of beef cytochrome c when presented with I-A(d). AODK-10.4 responded to keyhole limpet hemocyanin (KLH) presented with I-A (d). AODK-1.16 also responded to KLH presented by a product of the I region of H-2(d), but the data were consistent with either a product of the I-J-I-E(d) region or a combinatorial molecule with elements from both I-A(d) and I-E(d)/I-C(d). Coincidentally, AO-40.10 was shown to have an unexpected alloreactivity with a product of H-2(b) mapping to the K-I-A region. These hybrids should prove invaluable as sources of monoclonal material for the study of the receptor(s) on T cells with H-2-restricted antigen specificities. We also generated T cell hybrids with two antigen/H-2 specificities by fusing an azaguanine-resistant clone of AO-40.10 to normal T cells with a different antigen/H-2 specificity. Many of the hybrids retained reactivity to OVA plus H-2(a) and to the second antigen/H-2 combination. None reacted to either OVA plus the second H-2 type or to the second antigen plus H-2(a). One of these hybrids was successfully cloned to produce the line AOFK- 11.11.1. It retained the ability to recognize OVA plus I-A(k) inherited from one parent, and KLH plus IA(f) inherited from the other. It did not recognize OVA plus IA(f) or KLH plus I-A(k). These results have some bearing on models describing the nature of T cell receptors for antigen recognized in association with H-2 products. They do not support models in which antigen and H-2 are recognized separately by two independent T cell receptors.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-100572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-1078737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-1081575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-1085419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-11993330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-141046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-14978855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-214510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-305459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-306408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-307689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-307765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-308845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-308978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-320663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-417392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-4349479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-47901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-50400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-53263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-5499432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-55462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-567555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-6034749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-6154580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-6158551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-63988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-6447186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-6932473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-69515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-6966311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-6966321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-6968339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-70468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-77550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-84337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-91527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-91635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-92517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166712-92524
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
153
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1198-214
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pubmed:dateRevised |
2010-6-22
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pubmed:meshHeading |
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pubmed:year |
1981
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pubmed:articleTitle |
Antigen-inducible, H-2-restricted, interleukin-2-producing T cell hybridomas. Lack of independent antigen and H-2 recognition.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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