Linked Life Data

6166704

Source:http://linkedlifedata.com/resource/pubmed/id/6166704

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1981-9-15
pubmed:abstractText
The existence of T cells specific for soluble antigens in association with unique F(1) or recombinant major histocompatibility complex (MHC) gene products was first postulated from studies on the proliferative response of whole T cell populations to the antigen poly(Glu(55)Lys(36)Phe(9))(n) (GLphi). In this paper we use the newly developed technology of T lymphocyte cloning to establish unequivocally the existence of such cells specific for GLphi and to generalize their existence by showing that F(1)- specific cells can be isolated from T cell populations primed to poly(Glu(60)Ala(30)Tyr(10))(n) (GAT) where such clones represent only a minor subpopulation of cells. Gl.4b-primed B10.A(5R) and GAT-primed (B10.A x B10)F(1) lymph node T cells were cloned in soft agar, and the colonies that developed were picked and expanded in liquid culture. The GLphi-specific T cells were then recloned under conditions of high-plating efficiency to ensure that the final colonies originated from single cells. T cells from such rigorously cloned populations responded to stimulation with GILphi but only in the presence of nonimmune, irradiated spleen cells bearing (B10.A x B10)F(1) or the syngeneic B 10.A(5R) recombinant MHC haplotype. Spleen cells from either the B10 or B 10.A parental strains failed to support a proliferative response, even when added together. (B10 x B10.D2)F(1) and (B10 x B10.RIII)F(1) spleen cells also supported a proliferative response but (B10 x B10.Q)F(1) and (B10 X B10.S)F(1) spleen cells did not. These results suggested that the T cell clones were specific for GL[phi} in association with the beta(AE)(b)-alpha(E) (k,d,r,) Ia molecule and that recognition required both gene products to be expressed in the same antigen-presenting cells. Support for this interpretation was obtained from inhibition experiments using the monoclonal antibody Y-17 specific for a determinant on the beta(AE)(b)-alphaE Ia molecule. Y-17 completely inhibited the proliferative response of a GLphi-specific clone but had no effect on the response of either a PPD-specific or GAT-specific clone, both of which required the beta(A)-alpha(A) Ia molecule as their restriction element. No evidence could be found for the involvement of suppressor T cells in this inhibition. We therefore conclude that the phenomenon of F(1)-restricted recognition by proliferating T cells results from the presence of antigen- specific clones that must recognize unique F(1) or recombinant Ia molecules on the surface of antigen-presenting cells in addition to antigen in order to be stimulated.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-100572, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-1059157, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-1080781, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-1082491, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-1082919, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-1084902, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-1085312, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-1085419, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-233906, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-307765, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-308845, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-309484, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-310861, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-311437, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-429967, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-447366, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-447370, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-4538841, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-4572633, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-469258, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-47901, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-4817740, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-6153171, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-6153457, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-6158477, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-6158543, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-6158546, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-6158548, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-6158550, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-6161167, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-63988, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-6444235, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-6767788, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-6770025, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-6968269, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-6989911, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-70357, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-76333, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-77879, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-83698, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-87437, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-89077, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-91527, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-91645, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-92183, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-92520, http://linkedlifedata.com/resource/pubmed/commentcorrection/6166704-99319
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
153
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
677-93
pubmed:dateRevised
2010-6-22
pubmed:meshHeading
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