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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1981-8-20
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pubmed:abstractText |
Three criteria have been used to distinguish among different systems of embryonic cell adhesion: dependence on Ca2+, involvement of particular cell-surface molecules, and binding specificity. The characterization of the adhesion with respect to cell-surface molecules was carried out by using specific antibodies against the neural and liver cell adhesion molecules (N-CAM and L-CAM) and antibodies raised against retinal cells prepared by limited trypsinization in the presence of Ca2+ (called "T/Ca cells"). Aggregation of cells prepared from retina or brain without Ca2+ did not require Ca2+ and was inhibited by anti-(N-CAM) antibodies but not by anti-(L-CAM) or anti-T/Ca cell antibodies. In contrast, cells obtained from the same tissues in the presence of Ca2+ did require Ca2+ to aggregate. This aggregation was inhibited by anti-T/Ca cell antibodies but not by anti-(N-CAM) or anti-(L-CAM) antibodies. Hepatocyte aggregation also required Ca2+ and was inhibited only by anti-(L-CAM) antibodies. These results define three antigenically distinct cell adhesion systems in the embryo and raise the possibility that additional systems will be found. The neural Ca2+-independent system displayed a limited tissue specificity, mediating binding to neural but not liver cells. In contrast, the Ca2+-dependent systems of both neural and liver cells caused binding to all cell types tested. The Ca2+-dependent system was most active in retinal cells from 6-7 day embryos, whereas the Ca2+-independent system was most active at later times during development. In addition, treatments that inhibited the Ca2+-independent or Ca2+-dependent systems had very different effects on the fasciculation of neurites from dorsal root ganglia. All of the results suggest that Ca2+-independent and Ca2+-dependent adhesion systems play different functional roles during embryogenesis.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-1068472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-110634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-1141244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-264120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-4205052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-4351063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-4351182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-4424710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-456740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-457762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-5328643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-54921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-569154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-569155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-6933535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-7388946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-7391140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-7402293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-7430247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-769162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-812875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-893445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6165990-893446
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
387-91
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6165990-Animals,
pubmed-meshheading:6165990-Antigens,
pubmed-meshheading:6165990-Brain,
pubmed-meshheading:6165990-Calcium,
pubmed-meshheading:6165990-Cell Adhesion,
pubmed-meshheading:6165990-Cell Adhesion Molecules,
pubmed-meshheading:6165990-Chick Embryo,
pubmed-meshheading:6165990-Epitopes,
pubmed-meshheading:6165990-Ganglia, Spinal,
pubmed-meshheading:6165990-Liver,
pubmed-meshheading:6165990-Morphogenesis,
pubmed-meshheading:6165990-Retina,
pubmed-meshheading:6165990-Time Factors
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pubmed:year |
1981
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pubmed:articleTitle |
Distinct calcium-independent and calcium-dependent adhesion systems of chicken embryo cells.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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