Linked Life Data

6162154

Source:http://linkedlifedata.com/resource/pubmed/id/6162154

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
1981-4-13
pubmed:abstractText
The requirements for the decoding process at the ribosomal A site have been investigated in the presence of viomycin. For these studies natural mRNA was replaced either by the synthetic oligonucleotide A-U-G(-U)n, with 0 less than or equal to n less than or equal to 4, or by a physical mixture of the oligonucleotides A-U-G and various oligo(U) sequences. Thus the effect of the "removal" of selected covalent bonds from the sequence A-U-G(U)n could be studied. When the ribosomal P site contains tRNAMetf, then normally the full hexanucleotide "messenger" A-U-G-U-U-U is needed for the EF-Tu-mediated binding of Phe-tRNA into the A site. However in presence of viomycin the pentanucleotide A-U-G-U-U suffices for this. It is also possible in the presence of viomycin to replace A-U-G-U and U-U. In all the above systems the binding of Phe-tRNA required the presence of EF-Tu and GTP. The results suggest that viomycin reinforces interactions between aa-tRNA and the A site after the codon-anticodon recognition step.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6162154-14172630, http://linkedlifedata.com/resource/pubmed/commentcorrection/6162154-16591528, http://linkedlifedata.com/resource/pubmed/commentcorrection/6162154-183772, http://linkedlifedata.com/resource/pubmed/commentcorrection/6162154-202460, http://linkedlifedata.com/resource/pubmed/commentcorrection/6162154-209306, http://linkedlifedata.com/resource/pubmed/commentcorrection/6162154-212033, http://linkedlifedata.com/resource/pubmed/commentcorrection/6162154-4559253, http://linkedlifedata.com/resource/pubmed/commentcorrection/6162154-4563073, http://linkedlifedata.com/resource/pubmed/commentcorrection/6162154-4575689, http://linkedlifedata.com/resource/pubmed/commentcorrection/6162154-4580677, http://linkedlifedata.com/resource/pubmed/commentcorrection/6162154-4589304, http://linkedlifedata.com/resource/pubmed/commentcorrection/6162154-460419, http://linkedlifedata.com/resource/pubmed/commentcorrection/6162154-4902886, http://linkedlifedata.com/resource/pubmed/commentcorrection/6162154-4902905, http://linkedlifedata.com/resource/pubmed/commentcorrection/6162154-4945116, http://linkedlifedata.com/resource/pubmed/commentcorrection/6162154-6243944
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5813-24
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Dinucleotide codon-anticodon interaction as a minimum requirement for ribosomal aa-tRNA binding: stabilisation by viomycin of aa-tRNA in the A site.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't