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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1981-4-13
|
| pubmed:abstractText |
A simple method for the analysis of microbial proteases is described that was used to characterize the proteolytic activities of various Vibrio cholerae isolates. This method utilized the unique peptides generated from the degradation of a standard protein by proteases of various specificities. These peptides were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The unique patterns of peptides seen in gels can be used to type proteases according to their relative specificities. Culture supernatants of V. cholerae isolates from a variety of environmental and human sources were analyzed for the presence of a protease previously isolated and characterized in this laboratory from V. cholerae strain CA401. Supernatants from most isolates showing dimethyl casein proteolytic activity exhibited the presence of enzymes similar to the CA401 protease in their peptide digest patterns against bovine serum albumin and in their immunological reactivities. The probable widespread presence of this virulence-associated protease in V. cholerae isolates is discussed.
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| pubmed:grant | |
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6161944-320200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6161944-37166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6161944-415981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6161944-4215914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6161944-4326772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6161944-4996635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6161944-4999791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6161944-5920457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6161944-681794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6161944-7399688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6161944-819684
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0095-1137
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
13
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
80-4
|
| pubmed:dateRevised |
2009-11-18
|
| pubmed:meshHeading | |
| pubmed:year |
1981
|
| pubmed:articleTitle |
Characterization of Vibrio cholerae protease activities with peptide digest analysis.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|