Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1981-1-29
pubmed:abstractText
We applied the method of Guarente et al. [Guarente, L., Lauer, G., Roberts, T.M. & Ptashne, M. (1980) Cell 20, 543-553] to construct plasmids that direct expression in Escherichia coli of the human fibroblast interferon (F-IF) gene. Two plasmids were recovered. One directs efficient synthesis of a protein whose primary sequence is that of pre-F-IF and the other, that of mature F-IF. Extracts of bacteria synthesizing mature F-IF display antiviral activity characteristic of human F-IF. This activity is lower than that expected from the differential rate of synthesis of the protein. We have detected no such activity in extracts of bacteria synthesizing pre-F-IF.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159642-1061154, http://linkedlifedata.com/resource/pubmed/commentcorrection/6159642-1069307, http://linkedlifedata.com/resource/pubmed/commentcorrection/6159642-13465720, http://linkedlifedata.com/resource/pubmed/commentcorrection/6159642-16590396, http://linkedlifedata.com/resource/pubmed/commentcorrection/6159642-230483, http://linkedlifedata.com/resource/pubmed/commentcorrection/6159642-232222, http://linkedlifedata.com/resource/pubmed/commentcorrection/6159642-340049, http://linkedlifedata.com/resource/pubmed/commentcorrection/6159642-368040, http://linkedlifedata.com/resource/pubmed/commentcorrection/6159642-370836, http://linkedlifedata.com/resource/pubmed/commentcorrection/6159642-4599917, http://linkedlifedata.com/resource/pubmed/commentcorrection/6159642-5233831, http://linkedlifedata.com/resource/pubmed/commentcorrection/6159642-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/6159642-6157601, http://linkedlifedata.com/resource/pubmed/commentcorrection/6159642-6159625, http://linkedlifedata.com/resource/pubmed/commentcorrection/6159642-6248249, http://linkedlifedata.com/resource/pubmed/commentcorrection/6159642-6987533, http://linkedlifedata.com/resource/pubmed/commentcorrection/6159642-6990419, http://linkedlifedata.com/resource/pubmed/commentcorrection/6159642-7352259
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
77
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5230-3
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Expression of the human fibroblast interferon gene in Escherichia coli.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't