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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
1981-1-16
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pubmed:databankReference |
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pubmed:abstractText |
A cDNA library was constructed using mRNA from human fibroblasts induced with poly(I):poly(C). A bacterial clone containing fibroblast interferon cDNA sequences was identified by hybridization to a cDNA probe synthesized using deoxyoligonucleotide primers which hybridize to fibroblast interferon mRNA specifically. Expression plasmids were constructed which permitted the synthesis in E. coli of 8 x 10(7) units of human fibroblast interferon per liter of culture. The bacterially produced fibroblast interferon is indistinguishable from authentic human fibroblast interferon by several criteria.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-1060071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-1061154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-1069307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-1105573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-1252084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-183818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-265546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-269391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-282602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-306682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-360074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-386136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-4336693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-4610576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-4905667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-4939175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-503860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-6157094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-6157601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-6159597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-670186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-7352259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-7433092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-847463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6159584-85300
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0305-1048
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4057-74
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6159584-Amino Acid Sequence,
pubmed-meshheading:6159584-Base Sequence,
pubmed-meshheading:6159584-Cloning, Molecular,
pubmed-meshheading:6159584-DNA, Recombinant,
pubmed-meshheading:6159584-Escherichia coli,
pubmed-meshheading:6159584-Fibroblasts,
pubmed-meshheading:6159584-Humans,
pubmed-meshheading:6159584-Interferons,
pubmed-meshheading:6159584-Nucleic Acid Hybridization,
pubmed-meshheading:6159584-Protein Biosynthesis,
pubmed-meshheading:6159584-RNA, Messenger,
pubmed-meshheading:6159584-Transcription, Genetic
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pubmed:year |
1980
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pubmed:articleTitle |
Synthesis of human fibroblast interferon by E. coli.
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pubmed:publicationType |
Journal Article
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