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We have sequenced 3SE RNA, an unmodified species from hamster cell mitochondria that may be a 5S rRNA-equivalent. The sequence is [[Formula: see text]. The underlined stretches can form the stems of 2 hairpins whose existence is supported by S1 nuclease analysis. Residues 24 through 34 can also base-pair extensively with a sequence in the 3'-region of the small subunit ("13S") mitochondrial rRNA. These interactions resemble interactions postulated for 5S RNA.
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