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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1980-12-16
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pubmed:abstractText |
Studies in our laboratory and elsewhere have shown that it is possible to propagate antigen-specific murine T cells in vitro with resultant specific stepwise enrichment of antigen-induced proliferative cells. The proliferative responses of these T cells are antigen specific and dependent upon the presence of antigen-presenting cells (spleen cells) that share the I-A subregion with the proliferating T cell. Using techniques of soft-agar cloning, it has been further possible to isolate clones of antigen-reactive T lymphocytes from such long-term cultures. Data suggesting that these were clones of antigen-reactive T cells were obtained by studying the recognition of antigen in association with antigen-presenting cells with a panel of such clones of antigen-reactive T cells. Proof of clonality was obtained by subcloning. Clones derived from F1-immune mice can be divided into three separate categories: one clone recognizes antigen in association with antigen-presenting determinants of parent A and the F1; the second type recognizes antigen in association with antigen-presenting determinants of parent B and the F1; and the third type recognizes antigen only in association with antigen-presenting determinants of the F1 mouse. Genetic studies on the major histocompatibility complex requirements for antigen presentation to such F1-reactive T cell clones suggests that the hybrid antigen-presenting determinant in this system results from transcomplementation of products of the I-A region of haplotypes a and b. These studies support the concept developed in our laboratory that there exist unique F1 hybrid determinants on (A/J X C57BL/6) F1 cells and suggest that these determinants can be utilized physiologically by hybrid mice in immunocompetent cellular interactions.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-1082491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-112037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-115958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-145543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-162150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-302178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-306067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-307689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-308439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-308978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-309478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-309489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-310861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-411873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-4278109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-5220862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-52677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-6153457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-6158477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-66282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-6989911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-70357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-70468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-7359084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-77879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-78942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-84337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-91527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-91635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-92524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6158548-93286
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
152
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
759-70
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6158548-Animals,
pubmed-meshheading:6158548-Clone Cells,
pubmed-meshheading:6158548-Epitopes,
pubmed-meshheading:6158548-Female,
pubmed-meshheading:6158548-Genes, MHC Class II,
pubmed-meshheading:6158548-Histocompatibility Antigens Class II,
pubmed-meshheading:6158548-Hybridization, Genetic,
pubmed-meshheading:6158548-Lymphocyte Activation,
pubmed-meshheading:6158548-Major Histocompatibility Complex,
pubmed-meshheading:6158548-Male,
pubmed-meshheading:6158548-Mice,
pubmed-meshheading:6158548-Mice, Inbred Strains,
pubmed-meshheading:6158548-Recombination, Genetic,
pubmed-meshheading:6158548-T-Lymphocytes
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pubmed:year |
1980
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pubmed:articleTitle |
Antigen-reactive T cell clones. I. Transcomplementing hybrid I-A-region gene products function effectively in antigen presentation.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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