Linked Life Data

6158364

Source:http://linkedlifedata.com/resource/pubmed/id/6158364

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1980-12-18
pubmed:abstractText
The occurrence of insulin receptors and biological responses to insulin has been investigated in trypsin-dissociated fetal rat brain cells maintained in culture for 8 days. Binding of [125I]insulin to brain cells in culture was time- and pH-dependent and 85--90% specific. Porcine insulin competed for [125I]insulin binding in a dose-dependent manner. Unrelated polypeptides, including angiotensin II, glucagon, bovine growth hormone, and bovine prolactin did not compete for [125I]insulin binding. The half-life of [125I]insulin dissociation from receptors at 24 degrees C was 15 min and a plot of In[B/Bo] vs time suggested two dissociated rate constants of 2.7 X 10(-4) sec-1 and 5.0 X 10(-5) sec-1. Scatchard analysis of the binding data gave a curvilinear plot which may indicate negative cooperativity or the occurrence of both high affinity (Ka = 2 X 10(11) M-1) and low affinity (Ka = 4 X 10(10) M-1) sites. Of the estimated total of 4.9 X 10(4) binding sites per cell, 28--30% appear to be high affinity sites. Incubation of cultures with insulin caused a time- and dose-dependent stimulation of [3H]thymidine and [3H]uridine incorporation into TCA-precipitable material. Maximum stimulation of thymidine incorporation (2--5-fold) occurred 11 h after incubation with 167 nM insulin. The same concentration of insulin caused a 2.2-fold increase in [3H]uridine incorporation in 2 h. These results indicate that cells cultured from rat brain contain specific insulin receptors capable of mediating effects of insulin on macromolecular synthesis in the central nervous system.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
200
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
389-400
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Binding of [125I]insulin to specific receptors and stimulation of nucleotide incorporation in cells cultured from rat brain.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.