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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3-4
|
| pubmed:dateCreated |
1978-9-29
|
| pubmed:abstractText |
Pyruvate kinase deficiency was found to be the causative factor in a family with nonspherocytic hemolytic anemia. Biochemical findings were the following: decrease in adenosine-5'-triphosphate (ATP) concentration (59 micromoles per 100 ml of erytrocytes; normal: 150 +/- 59), increase in 2,3-diphosphoglycerate (2,3-DPG) concentration (13611 micromoles per 100 ml of erythrocytes; normal: 480 +/- 80) and low level of pyruvate kinase activity (955 U) mmol Hb; normal 2700 +/- 498. The deficiency of enzyme activity in the hexose-monophosphate shunt was excluded on bases of normal or elevated glucose-6-phosphate and 6-phosphogluconate dehydrogenase activities, normal reduced glutathione concentration and normal glutathione stability in vitro. The Michaelis constant for phosphoenolpyruvate of the patient's pyruvate kinase was found to be normal; thus, the mutation affected only the Vmax of the enzyme.
|
| pubmed:language |
hrv
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0350-2023
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
5
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
95-6
|
| pubmed:dateRevised |
2009-11-9
|
| pubmed:meshHeading | |
| pubmed:year |
1977
|
| pubmed:articleTitle |
[Deficiency of pyruvate kinase in erythrocytes: biochemical studies. Preliminary communication].
|
| pubmed:publicationType |
Journal Article,
English Abstract,
Case Reports
|