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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1980-7-22
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pubmed:abstractText |
An enzyme present in rabbit liver microsomes has been found to catalyze mannosyltransfer from GDP-mannose to exogenously added oligosaccharide-lipid acceptor resulting in the formation of an alpha-1,2-mannosyl-mannose linkage. Several lines of evidence suggest that the product is a heptasaccharide containing a single 1,6-linked branched glycosyl unit with 65% of the newly added mannosyl units in a terminal nonreducing position. The enzyme has been solubilized with non-ionic detergents and was partially purified on DEAE-cellulose and hydroxylapatite. The partially purified enzyme no longer catalyzed the formation of dolichol-P-mannose indicating a direct transfer from GDP-mannose to oligosaccharide-lipid acceptor.
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pubmed:language |
eng
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pubmed:journal |
|
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9258
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|
pubmed:author |
|
|
pubmed:issnType |
Print
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|
pubmed:day |
10
|
|
pubmed:volume |
255
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
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pubmed:pagination |
4170-5
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|
pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
|
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pubmed:year |
1980
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|
pubmed:articleTitle |
The biosynthesis of oligosaccharide-lipids. Formation of an alpha-1,2-mannosyl-mannose linkage.
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|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
