|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
22
|
|
pubmed:dateCreated |
1985-1-2
|
|
pubmed:abstractText |
[3H]Cytochalasin B was used as a ligand to identify and characterize the glucose transporter in cerebral microvessels of the rat and the pig. Specific cytochalasin B binding, defined as that fraction of the total binding that is stereospecifically displaced by excess (500 mM) D-glucose, is saturable. Kinetic studies of this specific binding to cerebral microvessel preparations showed a dissociation constant (Kd) of 0.65-0.88 microM and a maximal binding (Bmax) of 60-80 pmol/mg of protein. In comparison, the Bmax of particulate fractions of the cerebral cortex was about one-tenth that of cerebral microvessels. The ability of various hexoses to displace specific cytochalasin B binding to cerebral microvessels in vitro correlated well with the capability of these hexoses to cross the blood-brain barrier in vivo. Irreversible photoaffinity labeling of the glucose transporter of cerebral microvessels with cytochalasin B followed by solubilization and polyacrylamide gel electrophoresis labeled a polypeptide(s) with a molecular weight of about 53,000. Antibodies prepared against the glucose transporter of human erythrocytes also reacted with a polypeptide(s) with a molecular weight of about 53,000 on electrophoresed preparations of cerebral microvessels. These results indicate that cerebral microvessels are richly endowed with a glucose transporter moiety of similar molecular weight and antigenic characteristics as the glucose transporter of human erythrocytes and other mammalian tissues.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-1125240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-1151383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-14253434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-375257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-434144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-4580109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-469532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-4968036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-5866278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-6033532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-6108994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-6280190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-6289825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-6301198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-6311978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-6338925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-6361813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-6539126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-6619870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-6681949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-6685458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-6891216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-6954540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-6998256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-7014557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-7046746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-7046752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-711732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-7196262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-7200980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-7295669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-7328147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-73470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150484-921993
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
81
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
7233-7
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:6150484-Animals,
pubmed-meshheading:6150484-Blood-Brain Barrier,
pubmed-meshheading:6150484-Carrier Proteins,
pubmed-meshheading:6150484-Cerebral Cortex,
pubmed-meshheading:6150484-Cerebrovascular Circulation,
pubmed-meshheading:6150484-Cytochalasin B,
pubmed-meshheading:6150484-Deoxyglucose,
pubmed-meshheading:6150484-Glucose,
pubmed-meshheading:6150484-Male,
pubmed-meshheading:6150484-Molecular Weight,
pubmed-meshheading:6150484-Monosaccharide Transport Proteins,
pubmed-meshheading:6150484-Rats,
pubmed-meshheading:6150484-Rats, Inbred Strains,
pubmed-meshheading:6150484-Subcellular Fractions,
pubmed-meshheading:6150484-gamma-Glutamyltransferase
|
|
pubmed:year |
1984
|
|
pubmed:articleTitle |
Identification and characterization of the glucose transporter of the blood-brain barrier by cytochalasin B binding and immunological reactivity.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
