Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1984-12-31
pubmed:abstractText
We confirmed that the fimbriae of Haemophilus influenzae type b conferred hemagglutinating activity (HA) towards human erythrocytes, and erythrocytes of certain other species. Most (17/25) cerebrospinal fluid isolates lacked detectable HA on direct testing, but selective enrichment for fimbriation (f+) indicated that 22 of 25 strains could produce these surface structures. HA was unchanged from pH 4.5 to 9.5 and was not inhibited by mannose or certain other simple sugars. The HA titer of a suspension of three f+ strains was slightly decreased at 50 degrees C; HA was lost by heating at 60 degrees C for 3 min. Growth on a variety of solid and liquid media and under differing degrees of oxygenation did not change the HA titer of a suspension of three f+ strains. Fimbriation was not lost on repeated subculture. Wild-type fimbriated strains, and those derived by transformation, did not contain detectable plasmid DNA. Transformation of a strain lacking fimbriae to f+ was associated with the appearance of an outer membrane protein of 24 kilodaltons. This protein was purified from one strain to homogeneity on sodium dodecyl sulfate-polyacrylamide gel electrophoresis by selective detergent solubilization and ammonium sulfate fractionation. Colonization capacity was equivalent with an isogenic untypable strain lacking or possessing fimbriae. Fimbriae of type b H. influenzae possess characteristics similar to those structures on other gram-negative bacteria; their role in cell physiology or pathogenesis of invasive disease is unknown.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-1085778, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-110692, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-348723, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-4197389, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-4198720, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-4200266, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-4402481, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-4542033, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-5334095, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-603028, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-6103871, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-6106042, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-6109710, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-6116723, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-6126509, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-6127165, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-6127463, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-6128303, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-6138383, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-6138390, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-6139338, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-6286603, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-6286714, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-6976326, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-6977529, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-772168, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-809353, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-821935, http://linkedlifedata.com/resource/pubmed/commentcorrection/6150012-96134
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
787-96
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Characterization of Haemophilus influenzae type b fimbriae.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't