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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1984-11-13
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pubmed:abstractText |
Cultured endothelial cells from human umbilical cord labeled with [3H]20:4 release radiolabel when exposed to leukotrienes C or D (LTC or LTD). The major radiolabeled 20:4 metabolite recovered in the culture medium was prostacyclin. Both leukotrienes produced a dose-dependent synthesis of prostacyclin, with a maximal response at 10(-7) M leukotriene. LTC promoted a twofold greater response than did LTD at all concentrations tested (10(-9) to 10(-7) M). In contrast, no release of radiolabel above basal levels was evident with a challenge of LTE or LTB at the same concentrations. Endothelial cells metabolize approximately 40-50% of exogenously supplied LTC to LTD and LTE in 60 min. Levels of alpha-glutamyltranspeptidase (gamma-GTPase), the ectoenzyme reported to convert LTC or LTD, were detected in intact endothelial cells with the chromogenic substrate L-gamma-glutamyl-p-nitroanilide at levels sufficient to account for the observed rate of LTC metabolism. High concentrations of the gamma-GTPase inhibitors, glutathione and AT-125, blocked the metabolism of LTC by endothelium. These results suggest that degradation of leukotrienes by endothelium may be one mechanism for inactivation of these lipid mediators.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-34150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-371730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-4154944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-43155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-4355998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-4583980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-4940429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-5773262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-6102405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-6108512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-6109281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-6116705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-6120989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-6122208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-6136038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-6267608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-6283842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-6408642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-6799832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-6806265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-6894214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-6989368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6148375-7018698
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
160
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1043-53
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6148375-Arachidonic Acid,
pubmed-meshheading:6148375-Arachidonic Acids,
pubmed-meshheading:6148375-Cells, Cultured,
pubmed-meshheading:6148375-Endothelium,
pubmed-meshheading:6148375-Epoprostenol,
pubmed-meshheading:6148375-Histamine,
pubmed-meshheading:6148375-Humans,
pubmed-meshheading:6148375-Kinetics,
pubmed-meshheading:6148375-Leukotriene B4,
pubmed-meshheading:6148375-Leukotriene E4,
pubmed-meshheading:6148375-SRS-A,
pubmed-meshheading:6148375-Umbilical Veins,
pubmed-meshheading:6148375-gamma-Glutamyltransferase
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pubmed:year |
1984
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pubmed:articleTitle |
Stimulation of human endothelial cell prostacyclin synthesis by select leukotrienes.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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