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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
19
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pubmed:dateCreated |
1984-11-14
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pubmed:abstractText |
A N-ethylmaleimide-sensitive ATPase was extracted and partially purified from clathrin-coated vesicles of bovine brain. During purification the enzyme lost activity which was restored by a purified phospholipid fraction from brain. Phosphatidylserine, but no other commercial phospholipids tested, replaced the brain lipid fraction as activator. Particles depleted of the ATPase exhibited no H+ pump activity when reconstituted with brain phospholipids by the cholate dilution procedure. H+ pump activity was restored by incubating the reconstituted vesicles with the partially purified ATPase.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
|
pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
10
|
pubmed:volume |
259
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
11676-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:6148341-Animals,
pubmed-meshheading:6148341-Brain,
pubmed-meshheading:6148341-Cattle,
pubmed-meshheading:6148341-Clathrin,
pubmed-meshheading:6148341-Coated Pits, Cell-Membrane,
pubmed-meshheading:6148341-Endosomes,
pubmed-meshheading:6148341-Enzyme Activation,
pubmed-meshheading:6148341-Ethylmaleimide,
pubmed-meshheading:6148341-Phosphatidylserines,
pubmed-meshheading:6148341-Proton-Translocating ATPases
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pubmed:year |
1984
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pubmed:articleTitle |
Activation and partial purification of the ATPase of clathrin-coated vesicles and reconstitution of the proton pump.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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