Linked Life Data

6141616

Source:http://linkedlifedata.com/resource/pubmed/id/6141616

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4-6
pubmed:dateCreated
1984-3-8
pubmed:abstractText
In an attempt to delineate the mechanism subserving the demonstrated central effects of the tripeptide, L-prolyl-L-leucyl-glycinamide (PLG) in mammals including humans, we developed a radioligand binding assay to characterize the binding of 3H-PLG to rat brain membranes. Equilibrium binding studies indicated that PLG binds to rat striatum with high affinity (KD = 4.69 +/- 0.50 nM), saturability (Bmax = 9.20 +/- 0.30 fmoles mg-1 protein) and reversibility. Kinetic data yielded a KD = 1.42 +/- 0.21 nM for rat striatum. Regional distribution profile of specific 3H-PLG binding revealed that the striatum has the highest density of PLG binding sites, followed by the hypothalamus and the cerebral cortex. Analogues of PLG compete for specific PLG binding in rat striatum with potencies parallelling their in vivo activities in behavioural systems. Our results support the existence of a unique class of putative peptide receptor sites specific for PLG mediating a spectrum of pharmacological effects.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0278-5846
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
739-42
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Are the pharmacological effects of L-prolyl-L-leucyl-glycinamide (PLG) mediated through specific receptor mechanisms?
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't