Linked Life Data

6139841

Source:http://linkedlifedata.com/resource/pubmed/id/6139841

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1984-1-27
pubmed:abstractText
Immunocytochemistry enables the localization of transmitter-related antigens in tissue sections at either the light microscopic or the electron microscopic level. In the case of neuropeptides and certain transmitters (e.g. serotonin) it has been possible to produce antibodies directed against the putative transmitter itself. In other cases it has not been possible to produce useful antibodies against transmitters but antibodies have been raised against enzymes involved in transmitter metabolism (e.g. tyrosine hydroxylase, glutamic acid decarboxylase) and these antibodies are suitable markers for transmitter systems. Successful immunostaining with an antibody depends on a number of factors, two of the most important being the fixation of the antigen in the tissue and the visualization of the primary antibody once it has bound to the antigen. Techniques available for the visualization of bound primary antibody include the indirect-labelled immunofluorescence procedure and the unlabelled peroxidase-antiperoxidase (PAP) procedure. Direct-labelled immunocytochemistry is not now widely used but is likely to become increasingly important with the introduction of monoclonal antibodies and the development of techniques for the simultaneous localization of multiple antigens. Monoclonal antibody procedures also allow the production of antibodies against antigens which are difficult to purify such as certain transmitter markers (e.g. choline acetyltransferase) and constituents of neuronal membranes. Immunocytochemistry allows the production of detailed maps of the distribution of putative transmitters in the nervous system and in combination with tract tracing procedures is being used increasingly to identify transmitters in neuronal circuits. It has also been important in establishing the transmitter status of various neuroactive compounds in single neurones. Immunocytochemistry can be carried out on post-mortem samples and is providing information on transmitter distribution in normal and abnormal human brain.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0144-8757
pubmed:author
pubmed:issnType
Print
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
545-78
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Immunocytochemistry and neurobiology.
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't