Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
|
pubmed:dateCreated |
1983-12-21
|
pubmed:abstractText |
The repartition and characteristics of liver microsomal cytochrome P-450 fractions from benzene- or ethanol-treated rats were compared to those observed either in untreated animals, or in rats treated by classic inducers, namely phenobarbital, 3-methylcholanthrene, or beta-naphthoflavone. DEAE-cellulose chromatography allowed the separation of four main cytochrome P-450 fractions called A (nonabsorbed), Ba, Bb, and Bc (successively eluted by a NaCl gradient). In control rats, and in ethanol- and benzene-treated animals, fractions A were predominant; phenobarbital, 3-methylcholanthrene, beta-naphthoflavone, and benzene induced Bb fractions. Enzymatic and immunological methods allowed a characterization of those cytochrome P-450 fractions. Fractions A are similar in all cases, and mainly active towards aniline. This aniline hydroxylase activity is especially increased by ethanol. As a rule, fractions Ba and Bc behave similarly and exhibit rather low monooxygenase activities. On the contrary, fractions Bb differ from each other as a function of the inducer. Phenobarbital-induced Bb fraction is different from all other Bb fractions and especially active towards benzphetamine. 3-Methylcholanthrene- and beta-naphthoflavone-induced Bb fractions are identical, but they are different from all other Bb fractions, and especially active towards 7-ethoxycoumarin. Fraction Bb induced by benzene is different from those induced by classic inducers, but may be identical to the Bb fraction of control animals. As a whole, benzene and ethanol appear to display inducing properties different from those of phenobarbital or polycyclic aromatic hydrocarbon-like inducers.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Benzene,
http://linkedlifedata.com/resource/pubmed/chemical/Benzoflavones,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/Ethanol,
http://linkedlifedata.com/resource/pubmed/chemical/NADPH-Ferrihemoprotein Reductase,
http://linkedlifedata.com/resource/pubmed/chemical/Phenobarbital,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Naphthoflavone
|
pubmed:status |
MEDLINE
|
pubmed:issn |
0090-9556
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
11
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
499-506
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:6138238-Animals,
pubmed-meshheading:6138238-Benzene,
pubmed-meshheading:6138238-Benzoflavones,
pubmed-meshheading:6138238-Chromatography, DEAE-Cellulose,
pubmed-meshheading:6138238-Cytochrome P-450 Enzyme System,
pubmed-meshheading:6138238-Enzyme Induction,
pubmed-meshheading:6138238-Ethanol,
pubmed-meshheading:6138238-Immunodiffusion,
pubmed-meshheading:6138238-Male,
pubmed-meshheading:6138238-Microsomes, Liver,
pubmed-meshheading:6138238-NADPH-Ferrihemoprotein Reductase,
pubmed-meshheading:6138238-Phenobarbital,
pubmed-meshheading:6138238-Radioimmunoassay,
pubmed-meshheading:6138238-Rats,
pubmed-meshheading:6138238-Rats, Inbred Strains,
pubmed-meshheading:6138238-beta-Naphthoflavone
|
pubmed:articleTitle |
Influence of ethanol and benzene on cytochrome P-450 fractions in rat liver microsomes.
|
pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|