Linked Life Data

6138227

Source:http://linkedlifedata.com/resource/pubmed/id/6138227

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1983-12-21
pubmed:abstractText
Steroid glucuronidation was investigated in solubilized female rat and rabbit liver microsomes and in preparations of UDP-glucuronsyltransferase (UDPGT) activity resolved from these organelles. Solubilized rabbit liver microsomes possessed relatively high UDPGT activity towards estrone and beta-estradiol but not testosterone. Glucuronidation observed at the 3-OH position of beta-estradiol is 20-fold greater than at the 17-OH position. Estrone UDPGT activity, highly purified from rabbit liver microsomes, was active towards estrone and the 3-OH position of beta-estradiol but not towards testosterone (17-OH) or the 17-OH position of beta-estradiol. Thus, estrone UDPGT activity demonstrated essentially the same specificity for conjugation of the 3-OH position of beta-estradiol as observed in microsomes. Solubilized liver microsomes from female rats possessed approximately 4-fold more activity towards testosterone (17-OH) than estrone (3-OH). Rat liver microsomes formed about 2.5-fold more beta-estradiol 17-glucuronide than 3-glucuronide. Following a Chromatofocusing procedure where a gradient from pH 9-7 was employed, an eluant fraction was obtained that was enriched in estrone UDPGT activity relative to testosterone UDPGT activity. The fraction preferentially conjugated the 3-OH position of beta-estradiol. Two eluant fractions were obtained which demonstrated high levels of UDPGT activity towards testosterone and beta-estradiol but not estrone. Both fractions possessed a UDPGT activity that displayed a high degree of specificity for conjugation of the 17-OH position of beta-estradiol. A 17-OH steroid UDPGT was purified to apparent homogeneity from one of these fractions. These results suggest that separate UDPGT activity exists in female rat liver for the glucuronidation of the 3- and 17-OH positions of beta-estradiol. In female rabbit liver, beta-estradiol is predominantly conjugated at the 3-OH position by a single form of UDPGT.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0090-9556
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
426-32
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:articleTitle
Steroid 3- and 17-OH UDP-glucuronosyltransferase activities in rat and rabbit liver microsomes.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, U.S. Gov't, P.H.S.