Linked Life Data

6135145

Source:http://linkedlifedata.com/resource/pubmed/id/6135145

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1983-8-11
pubmed:abstractText
The extent of the promethazine-sensitive binding of [3H]mepyramine to a washed fraction from guinea pig cerebellum was little altered between 4 degrees and 30 degrees. The dissociation of [3H]mepyramine from the H1 receptor was markedly temperature-sensitive. An Arrhenius plot of the variation of the dissociation rate constant, k-1, with temperature was linear over the range 37 degrees -15 degrees (E alpha = 160 kJ mole-1). The association rate constant, k1, was also temperature-dependent, and an Arrhenius plot approximated well to a straight line between 30 degrees and 15 degrees (E alpha = 112 kJ mole-1). The linear relationship may hold down to 4 degrees. A consequence of the slow dissociation at 4 degrees is that the IC50 for mepyramine inhibition of promethazine-sensitive [3H]mepyramine binding at 4 degrees is independent of the concentration of [3H]mepyramine if the cerebellar homogenate is first incubated with the nonradioactive antagonist before addition of the 3H-ligand. Promethazine binding showed a temperature dependence similar to that of mepyramine, and for both antagonists and for chlorpromazine the affinity constant was not greatly increased (equal to or less than a factor of 2) at 4 degrees compared with 30 degrees. Tripelennamine showed a more rapid dissociation at 4 degrees than the other antagonists. Mequitazine dissociated slowly at 4 degrees, but the affinity constant was lower at 4 degrees than at 30 degrees.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0026-895X
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
60-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Temperature dependence of the binding of [3H]mepyramine and related compounds to the histamine H1 receptor.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't