Linked Life Data

6134588

Source:http://linkedlifedata.com/resource/pubmed/id/6134588

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1983-8-26
pubmed:abstractText
Conditions have been developed which allow for the efficient, accurate, and specific polyadenylation of exogenously added mRNA precursors in a whole-cell lysate derived from HeLa cells. Precursors are prepared by in vitro transcription using linear DNA templates in a HeLa whole-cell lysate, purified, and added to another lysate. Under optimal conditions (which are quite precise with respect to several variables), 70% or more of the precursor molecules become polyadenylated, and the length of the poly(A) segment added is controlled much as it is in vivo, giving rise to 150-300-nucleotide long stretches of poly(A). Under suboptimal conditions, both the fraction of precursor RNA that becomes polyadenylated and also the length of the poly(A) segment added are reduced. The in vitro polyadenylation reaction is also remarkably specific: Only in vitro-synthesized pre-mRNAs that contain a 3' end located at or slightly downstream from the corresponding in vivo mRNA 3' end site can be efficiently polyadenylated in vitro. These results suggest that the poly(A) polymerase requires one or more protein (or RNA) factors in order to bring about accurate and specific polyadenylation in vitro, and that the poly(A) polymerase complex must interact with a specific recognition signal at the 3' end of mRNA precursors in order to catalyze subsequent polyadenylation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0092-8674
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
595-605
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Accurate and specific polyadenylation of mRNA precursors in a soluble whole-cell lysate.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.