Linked Life Data

6124969

Source:http://linkedlifedata.com/resource/pubmed/id/6124969

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1982-9-17
pubmed:abstractText
We have provided evidence for the existence of a biologically active opioid in toad (Bufo marinus) brain that is immunoreactive with antiserum raised against dynorphin (1-13). Compared with porcine dynorphin, this opioid is similar in apparent molecular weight on the basis of gel permeation chromatography and is more hydrophobic on the basis of high-performance liquid chromatography. After purification, its opioid biological activity was demonstrated on the guinea pig ileum myenteric plexus-longitudinal muscle preparation. It was found to be less potent, and to have a similar sensitivity to antagonism by naloxone, in comparison with porcine dynorphin. Because it is immunoreactive with antiserum specific for porcine dynorphin, it probably has considerable sequence homology. Generally, the tissue distribution of immunoreactive dynorphin in the toad is similar to that in the rat, with highest concentrations in the neurointermediate lobe of the pituitary. However, the anterior lobe of the toad pituitary contains considerably lower concentrations than are found in the rat anterior lobe. There appear to be three size classes of immunoreactive dynorphin in toad neural tissue, each with apparent molecular weight below 12,000, similar to the size classes of immunoreactive dynorphin found in pig and rat. However, in toad spinal cord (and possibly in brain) there is immunoreactive dynorphin of greater apparent molecular weight, which has not been reported in mammalian tissue. The contribution of each molecular size to the total immunoreactivity varies from tissue to tissue and is different from that observed in the rat.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6124969-14450081, http://linkedlifedata.com/resource/pubmed/commentcorrection/6124969-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/6124969-230519, http://linkedlifedata.com/resource/pubmed/commentcorrection/6124969-4799172, http://linkedlifedata.com/resource/pubmed/commentcorrection/6124969-6105608, http://linkedlifedata.com/resource/pubmed/commentcorrection/6124969-6108564, http://linkedlifedata.com/resource/pubmed/commentcorrection/6124969-6118865, http://linkedlifedata.com/resource/pubmed/commentcorrection/6124969-6118870, http://linkedlifedata.com/resource/pubmed/commentcorrection/6124969-6121324, http://linkedlifedata.com/resource/pubmed/commentcorrection/6124969-6133255, http://linkedlifedata.com/resource/pubmed/commentcorrection/6124969-7052485, http://linkedlifedata.com/resource/pubmed/commentcorrection/6124969-7247946
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3345-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
A dynorphin-like opioid in the central nervous system of an amphibian.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.