Linked Life Data

6123133

Source:http://linkedlifedata.com/resource/pubmed/id/6123133

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1982-7-19
pubmed:abstractText
A method is described for the preparation of an enriched population of proximal tubules from the cortices of neonate (21-28 d old) rabbits. The method uses collagenase-hyaluronidase digestion, followed by gentle shear to yield a suspension of tubules and glomeruli. Tubular enrichment is achieved by discontinuous density gradient centrifugation in a Percoll gradient. Two fractions were obtained by this method. The denser fraction contained predominantly proximal tubules, was depleted of glomeruli and was enriched in the proximal tubule marker enzyme alkaline phosphatase. Qualitatively similar results to those obtained with neonate animals were obtained with adult tissue. Neonate tubules from the denser gradient fraction grew readily in tissue culture. When examined by electron microscopy the cells exhibited a marked polarity of ultrastructural organization and retained apical tight junctions. Despite this, there was an obvious loss of structure in comparison with in vivo proximal tubule cells. The use of primary culture techniques to study in vitro renal epithelial function is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0144-8757
pubmed:author
pubmed:issnType
Print
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
287-301
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1982
pubmed:articleTitle
Isolation and culture of renal cortical tubules from neonate rabbit kidneys.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't