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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1982-2-12
|
| pubmed:abstractText |
We present a revised method for the preparation, storage and assay of the Cl--ATPase activity responsible for salt secretion in Limonium vulgare. Altering the centrifugation step improved the yield and linked-enzyme assay for ADP provided a sensitive method for continuous monitoring of Cl--ATPase activity. The activity of untreated membranes was low but fairly stable. Treatment with detergent gave strong stimulation of Cl--ATPase activity ATP between 0.1 and 0.2 mM. Cl- stimulated the activity up to a maximum at 0.13 M Cl-, and the pH optimum was around 6.3 to 6.4.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0006-3002
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
6
|
| pubmed:volume |
648
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
186-91
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:6118178-Adenosine Triphosphatases,
pubmed-meshheading:6118178-Anion Transport Proteins,
pubmed-meshheading:6118178-Cell Membrane,
pubmed-meshheading:6118178-Chlorides,
pubmed-meshheading:6118178-Hydrogen-Ion Concentration,
pubmed-meshheading:6118178-Kinetics,
pubmed-meshheading:6118178-Plants
|
| pubmed:year |
1981
|
| pubmed:articleTitle |
Improved preparation and assay and some characteristics of Cl--ATPase activity from Limonium vulgare.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|