Linked Life Data

6101202

Source:http://linkedlifedata.com/resource/pubmed/id/6101202

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3 Pt 2
pubmed:dateCreated
1993-9-22
pubmed:abstractText
Infection of NIH/3T3 and Balb/c/3T3 fibroblastic cell lines by Abelson virus results in stable transformation in most cases. We have observed that certain rodent cell lines, including some Balb/c/3T3 sublines, do not maintain stable transformation following infection. Within three to four weeks of infection of these cell lines a large fraction of the cells is not recoverable upon serial passage and appear to be killed. The residual population of cells displays a flat, nontransformed phenotype. Concomitant with this reversion is the loss of the integrated provirus and loss of expression of the Abelson virus transforming protein. This loss of the integrated provirus is specific to transformation-competent viral strains. The effect of Abelson virus on these cells appears to be, in part, due to a lethal effect of the transforming protein on the cells. We have selectively isolated mutants of Abelson virus that stably transform these rodent cell lines without expressing a lethal effect. Analysis of the nucleic acid and protein structure of these mutant virus strains showed that the transforming proteins are shorter, having lost expression of the carboxy-terminal one third of the protein. We suspect that the carboxy-terminal portion of the wild-type Abelson protein may modulate the in vivo expression of the protein kinase activity and that this may cause the transforming protein to have a deleterious effect on certain cell lines.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0092-8674
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
477-86
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Lethal effect of the Abelson murine leukemia virus transforming gene product.
pubmed:affiliation
Molecular Biology Institute, University of California, Los Angeles 90024.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't