rdf:type |
|
lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0017262,
umls-concept:C0022490,
umls-concept:C0032098,
umls-concept:C0086860,
umls-concept:C0185117,
umls-concept:C0439851,
umls-concept:C1456463,
umls-concept:C1510411,
umls-concept:C1552596,
umls-concept:C1947931,
umls-concept:C2911684
|
pubmed:issue |
6
|
pubmed:dateCreated |
1985-4-26
|
pubmed:abstractText |
The promoter region of the CaMV inclusion body protein gene was modified for use in chimeric gene fusions. The modified promoter was used to construct a selectable marker for plant transformation based on the Tn 5 kanamycin resistance gene. This chimeric selectable marker was introduced into plant cells using oncogenic and deoncogenized strains of Agrobacterium tumefaciens. Both types of transformation produced kanamycin-resistant cell lines. The resistant cell lines derived from the deoncogenized strains were used to regenerate shoots. A second type of selection based on the ability of octopine synthase to detoxify aminoethyl cysteine was also used to select transformants in both oncogenic and nononcogenic transformation.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:issn |
0271-6801
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
2
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
549-62
|
pubmed:dateRevised |
2008-11-21
|
pubmed:meshHeading |
pubmed-meshheading:6099400-Amino Acid Oxidoreductases,
pubmed-meshheading:6099400-Cell Transformation, Neoplastic,
pubmed-meshheading:6099400-Cloning, Molecular,
pubmed-meshheading:6099400-DNA, Viral,
pubmed-meshheading:6099400-Drug Resistance,
pubmed-meshheading:6099400-Escherichia coli,
pubmed-meshheading:6099400-Genes, Viral,
pubmed-meshheading:6099400-Kanamycin,
pubmed-meshheading:6099400-Kanamycin Kinase,
pubmed-meshheading:6099400-Mosaic Viruses,
pubmed-meshheading:6099400-Phosphotransferases,
pubmed-meshheading:6099400-Plants,
pubmed-meshheading:6099400-Promoter Regions, Genetic,
pubmed-meshheading:6099400-Rhizobium,
pubmed-meshheading:6099400-Transformation, Genetic
|
pubmed:year |
1984
|
pubmed:articleTitle |
A cauliflower mosaic virus promoter directs expression of kanamycin resistance in morphogenic transformed plant cells.
|
pubmed:publicationType |
Journal Article
|