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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-3
|
| pubmed:dateCreated |
1985-3-29
|
| pubmed:abstractText |
Derivatives of plasmid pBR322 carrying the tryptophan operon are rapidly lost from a growing culture. The valS gene from Escherichia coli was combined with the tryptophan operon on a pBR322 derivative, pSGS21. In a thermosensitive valS mutant of E. coli K-12 host, plasmid pSGS21 was stably inherited for over 200 generations at temperatures nonpermissive for the chromosomal mutation. At 30 degrees C, a temperature at which the host valSts protein is functional, the plasmid was lost at a rate of 1.2% per cell generation.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0378-1119
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
31
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
117-22
|
| pubmed:dateRevised |
2006-5-1
|
| pubmed:meshHeading |
pubmed-meshheading:6098518-Bacterial Proteins,
pubmed-meshheading:6098518-Cloning, Molecular,
pubmed-meshheading:6098518-Escherichia coli,
pubmed-meshheading:6098518-Genes, Bacterial,
pubmed-meshheading:6098518-Genetic Vectors,
pubmed-meshheading:6098518-Operon,
pubmed-meshheading:6098518-Plasmids,
pubmed-meshheading:6098518-Temperature,
pubmed-meshheading:6098518-Tryptophan Synthase,
pubmed-meshheading:6098518-Valine-tRNA Ligase
|
| pubmed:year |
1984
|
| pubmed:articleTitle |
Temperature-dependent retention of a tryptophan-operon-bearing plasmid in Escherichia coli.
|
| pubmed:publicationType |
Journal Article
|