Linked Life Data

6097090

Source:http://linkedlifedata.com/resource/pubmed/id/6097090

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1985-2-11
pubmed:abstractText
Twenty gonococcal isolates representing different serovars of the protein IA & IB serogroups, as determined by co-agglutination with monoclonal antibodies, were investigated by the restriction enzyme (RE) technique with Hind III enzyme. The patterns resulting from RE digestion and subsequent electrophoresis of the DNA fragments in gel consisted of 40-45 bands. Eight RE patterns were observed among ten of the protein IA strains representing three serovars with an identical pattern for three strains of the same serovar with positive epidemiology. Seven RE patterns were observed among ten of the protein IB strains representing five serovars with identical patterns for each of three pairs with available positive epidemiology for two of them. Seven samples from the same bacterial clone extracted and digested separately showed identical band patterns. The genetic stability was illustrated by identical patterns through 41 passages in vitro and by the same band pattern of the various virulent and avirulent colony morphology variants of a particular gonococcal strain. The results show that RE analysis of the gonococcal DNA genome is well suited as an adjunct to phenotypic markers in epidemiological studies of gonorrhoea.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0108-0180
pubmed:author
pubmed:issnType
Print
pubmed:volume
92
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
271-8
pubmed:dateRevised
2009-6-4
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Restriction endonuclease fingerprinting of chromosomal DNA of Neisseria gonorrhoeae.
pubmed:publicationType
Journal Article