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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1985-2-5
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pubmed:abstractText |
This paper describes a mutator system in the nematode Caenorhabditis elegans var. Bergerac for the gene unc-22. Of nine C. elegans and two C. briggsae strains tested only the Bergerac BO strain yielded mutant animals at a high frequency and the unc-22 IV gene is a preferred mutational target. The forward spontaneous mutation frequency at the unc-22 locus in Bergerac BO is about 1 X 10(-4), and most of these spontaneous unc-22 mutations revert at frequencies between 2 X 10(-3) and 2 X 10(-4). Both the forward mutation frequency and the reversion frequency are sensitive to genetic background. Spontaneous unc-22 mutations derived in a Bergerac background and placed in a primarily Bristol background revert at frequencies of less than 10(-6). When reintroduced into a Bergerac/Bristol hybrid background the mutations once again become unstable. The mutator activity could not be localized to a discrete site in the Bergerac genome. Nor did mutator activity require the Bergerac unc-22 gene as a target since the Bristol unc-22 homolog placed in a Bergerac background also showed high mutation frequency. Intragenic mapping of two spontaneous unc-22 alleles, st136 and st137, place both mutations in the central region of the known unc-22 map. However, these mutations probably recombine with one another, suggesting that the unstable mutations can occur in more than one site in unc-22. Examination of the phenotypic effect of these mutations on muscle structure indicates that they are less severe in their effect than a known amber allele. We suggest that this mutator system is polygenic and dispersed over the nematode genome and could represent activity of the transposable element Tc1.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-13433592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-16587134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-16592430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-17246216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-17246266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-17248882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-17796454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-322276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-378399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-5115378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-6059362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-6251544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-6260015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-6260376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-6264444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-6289435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-6289436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-6290083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-6302521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-6306578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-6320712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-6321037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-6368313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-7120406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-7173602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-7187364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-7190524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6096205-7250500
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0016-6731
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
108
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
859-77
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
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pubmed:year |
1984
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pubmed:articleTitle |
Spontaneous unstable unc-22 IV mutations in C. elegans var. Bergerac.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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