Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1985-1-2
|
pubmed:abstractText |
Cytochrome c oxidase preparations from bovine heart muscle contain 1 zinc per 2 irons. Metal contents of nine preparations determined by inductively coupled plasma atomic emission spectrometry (ICP-AES) show that Cu, Fe and Zn are the only metals present in significant amounts with average Cu/Fe, Fe/Zn, and Cu/Zn atom ratios of 1.3, 2.1 and 2.8, respectively. Removal of adventitious copper results in a Cu:Fe:Zn stoichiometry of 2:2:1. The zinc is tightly bound. Dialysis against a solution of 1,10-phenanthroline at pH 7.4 or an acidic buffer (pH 4.4) does not remove Zn. Dialysis against 0.8 M KCN at pH 10 causes partial loss of both Cu and Zn. This is the first evidence for the presence of Zn in a cytochrome c oxidase.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Nov
|
pubmed:issn |
0006-291X
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
14
|
pubmed:volume |
124
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
836-42
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:6095824-Animals,
pubmed-meshheading:6095824-Cattle,
pubmed-meshheading:6095824-Copper,
pubmed-meshheading:6095824-Electron Transport Complex IV,
pubmed-meshheading:6095824-Iron,
pubmed-meshheading:6095824-Myocardium,
pubmed-meshheading:6095824-Spectrophotometry, Atomic,
pubmed-meshheading:6095824-Zinc
|
pubmed:year |
1984
|
pubmed:articleTitle |
Zinc is a constituent of bovine heart cytochrome c oxidase preparations.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|