rdf:type |
|
lifeskim:mentions |
umls-concept:C0022942,
umls-concept:C0027021,
umls-concept:C0027950,
umls-concept:C0030685,
umls-concept:C0032102,
umls-concept:C0038164,
umls-concept:C0205171,
umls-concept:C0391871,
umls-concept:C0680255,
umls-concept:C1283071,
umls-concept:C1511790,
umls-concept:C1963578
|
pubmed:issue |
4
|
pubmed:dateCreated |
1985-1-10
|
pubmed:abstractText |
A haemolytic plaque assay was adopted to detect release of lactoferrin and myeloperoxidase (MPO) from single neutrophils. Target erythrocytes coated with protein A were bound as a monolayer by poly-L-lysine to the surface of a plastic dish. Secreted lactoferrin and MPO induced plaque formation dependent on the reaction with complement and specific antiserum producing lysis of the protein-A-coated sheep red blood cells. Lactoferrin was found to be released spontaneously from a fraction of neutrophils while MPO was released only after phagocytosis, reflecting different mechanisms for degranulation of MPO-containing azurophil and lactoferrin-containing specific granules.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Oct
|
pubmed:issn |
0300-9475
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
20
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
349-53
|
pubmed:dateRevised |
2011-11-17
|
pubmed:meshHeading |
pubmed-meshheading:6095438-Antibody-Producing Cells,
pubmed-meshheading:6095438-Complement System Proteins,
pubmed-meshheading:6095438-Hemolytic Plaque Technique,
pubmed-meshheading:6095438-Humans,
pubmed-meshheading:6095438-Lactoferrin,
pubmed-meshheading:6095438-Lactoglobulins,
pubmed-meshheading:6095438-Neutrophils,
pubmed-meshheading:6095438-Peroxidase,
pubmed-meshheading:6095438-Peroxidases,
pubmed-meshheading:6095438-Phagocytosis,
pubmed-meshheading:6095438-Staphylococcal Protein A
|
pubmed:year |
1984
|
pubmed:articleTitle |
Detection of lactoferrin and myeloperoxidase release from single neutrophils by a protein A plaque assay.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|