Linked Life Data

6093591

Source:http://linkedlifedata.com/resource/pubmed/id/6093591

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5 Pt 2
pubmed:dateCreated
1984-12-12
pubmed:abstractText
The transport of sulfate into outer cortical brush border membrane vesicles (early proximal tubule) was studied. Sulfate uptake was markedly enhanced by sodium and slightly enhanced by lithium but showed no stimulation by other monovalent cations tested. Kinetic analysis of the sodium-dependent component of sulfate flux indicated a single transport system obeying Michaelis-Menten kinetics. Studies of the dependence of sulfate flux on sodium concentration indicated that more than one sodium ion was involved in the sulfate transport event. Although sulfate influx was found to be stimulated by negative intravesicular potentials, evidence is presented that sodium/sulfate cotransport is nevertheless an electroneutral event presumably involving 2 Na+ per SO2-4. Under zero trans sodium conditions sodium-dependent sulfate influx was stimulated by the presence of intravesicular unlabeled sulfate (trans stimulation); however, a similar stimulation of sulfate efflux by the presence of unlabeled extravesicular sulfate was not observed. These latter results are consistent with an ordered binding scheme for the transporter, with sulfate binding before sodium on the extravesicular (urine) surface and dissociating before sodium on the intravesicular (cytosolic) surface.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
247
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
F793-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Sodium-dependent sulfate transport in renal outer cortical brush border membrane vesicles.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't