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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1984-12-18
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pubmed:abstractText |
The interaction of the herpes simplex virus type 1 (HSV-1) major DNA-binding protein, infected-cell polypeptide 8 (ICP8), with nucleic acids has been examined by a filter-binding assay and electron microscopy. Filter-binding assays done over a broad pH range indicated that the optimum pH for the protein-DNA interaction is approximately 7.6. Heat inactivation studies showed that ICP8 is stable at temperatures up to 40 degrees C with a rapid loss of binding activity on incubation at 45 degrees C and above. Competition binding experiments have established the following relative affinities of ICP8 for the following nucleic acids: single-stranded HSV-1 DNA congruent to bacteriophage fd DNA greater than polyriboadenylate much greater than double-stranded HSV-1 DNA congruent to d(pCpT)5. Observation of negatively stained ICP8-single-stranded DNA complexes indicated that ICP8 binds along the length of the DNA in a regular repeating fashion. The average width of these complexes is 9.3 +/- 0.8 nms. Finally. Finally, addition of purified ICP8 to HSV-1 DNA polymerase reactions resulted in a stimulation of the viral polymerase activity.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-1090613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-10965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-1103970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-14402487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-171843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-180249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-183021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-185791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-188782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-207894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-21304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-4365321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-4566449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-5045301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-6171613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-6175245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-6270358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-6287005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-6292530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-6296442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-6302216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-6302328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-6304350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-6312079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-6764531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-731693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-791947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6092704-791948
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
52
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
727-33
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6092704-DNA, Single-Stranded,
pubmed-meshheading:6092704-DNA, Viral,
pubmed-meshheading:6092704-DNA-Binding Proteins,
pubmed-meshheading:6092704-DNA-Directed DNA Polymerase,
pubmed-meshheading:6092704-Hydrogen-Ion Concentration,
pubmed-meshheading:6092704-Protein Binding,
pubmed-meshheading:6092704-Simplexvirus,
pubmed-meshheading:6092704-Temperature,
pubmed-meshheading:6092704-Virus Replication
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pubmed:year |
1984
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pubmed:articleTitle |
Interaction with nucleic acids and stimulation of the viral DNA polymerase by the herpes simplex virus type 1 major DNA-binding protein.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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