Linked Life Data

6091257

Source:http://linkedlifedata.com/resource/pubmed/id/6091257

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1984-11-19
pubmed:abstractText
Transferrin-mediated uptake of iron has been studied in K-562 human erythroleukaemia cells. K-562 cells accumulate iron from transferrin by a temperature-dependent mechanism linearly with time for at least 150 min. At 37 degrees C the uptake reaches a saturation level of approximately 25 pmol iron/10(6) cells/h at a concentration of iron (as iron-transferrin) of 5 mumol/l. The binding of transferrin reveals saturation kinetics, at 0.2 mumol/l of transferrin specific binding amounts to 1.8 pmol/10(6) cells. Relative to the binding of transferrin the uptake of iron increases with time. The ability of K-562 cells to accumulate iron declines with days in culture by approximately 50% from day 1 to day 5. The results are compatible with iron uptake by receptor-mediated endocytosis of transferrin, dissociation of iron within the cell and exocytosis of apotransferrin.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0036-5513
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
549-53
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Binding of transferrin and uptake of iron by K-562 cells.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't