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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
1984-11-5
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pubmed:abstractText |
Ca2+-channel currents in primary cultures of bovine adrenal chromaffin cells were studied using the whole-cell patch-clamp method. Parameters of a double-pulse protocol were systematically varied to characterize facilitation by a prepulse (P1) of Ca2+-channel current during a test pulse (P2). The pulses were usually separated by 30 msec, an interval sufficient for decay of any measurable P1 tail currents. The Ca2+-channel current amplitude during P2 increased when P1 voltage was more positive than 0 mV. The effect became progressively greater with more positive P1 voltage. With a 60-msec P1 to +80 mV, the current amplitude typically increased by 25%-35% during a 60-msec P2. Comparison of facilitated and control inward Ca2+-channel current I(V) curves showed that facilitation was also strongly dependent on P2 test voltage. Facilitation of Ca2+-channel currents is a voltage-dependent phenomenon and is not dependent on Ca2+ entry. When short repetitive voltage-clamp pulses were applied, the Ca2+-channel current amplitude increased with each pulse. This suggests that Ca2+-channel facilitation could enhance release of catecholamines from chromaffin cells during a train of action potentials.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091117-266215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091117-4296698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091117-4296699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091117-4845658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091117-6050500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091117-6065887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091117-6137190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091117-6161228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091117-6261668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091117-6270629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091117-6284915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091117-6296371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091117-6296372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091117-6303205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091117-6420549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091117-7069629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091117-7238593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091117-917284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091117-994041
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
81
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5871-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1984
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pubmed:articleTitle |
Facilitation of Ca2+-channel currents in bovine adrenal chromaffin cells.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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