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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
1984-11-5
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pubmed:abstractText |
A cDNA library was prepared from a terminal deoxynucleotidyltransferase-containing thymoma in the lambda phage vector lambda gt11. By screening plaques with anti-terminal transferase antibody, positive clones were identified of which some had beta-galactosidase-cDNA fusion proteins identifiable after electrophoretic fractionation by immunoblotting with anti-terminal transferase antibody. The predominant class of cross-hybridizing clones was determined to represent cDNA for terminal transferase by showing that one representative clone hybridized to a 2200-nucleotide mRNA in close-matched enzyme-positive but not to enzyme-negative cells and that the cDNA selected a mRNA that translated to give a protein of the size and antigenic characteristics of terminal transferase. Only a small amount of genomic DNA hybridized to the longest available clone, indicating that the sequence is virtually unique in the mouse genome.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-1054106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-1085332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-1127379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-14459258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-311896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-368657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-4330042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-4530991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-4545077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-5543689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-6027247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-6175635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-6219389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-6251240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-6277505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-6286838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-6287227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-6287467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-6546606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-6601132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-6773666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-6788376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-7068615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-7231520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-7263682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-7356645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6091113-7425302
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
81
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5836-40
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6091113-Animals,
pubmed-meshheading:6091113-Antibodies,
pubmed-meshheading:6091113-Antigen-Antibody Complex,
pubmed-meshheading:6091113-Bacteriophage lambda,
pubmed-meshheading:6091113-Cloning, Molecular,
pubmed-meshheading:6091113-DNA,
pubmed-meshheading:6091113-DNA Nucleotidylexotransferase,
pubmed-meshheading:6091113-DNA Nucleotidyltransferases,
pubmed-meshheading:6091113-Escherichia coli,
pubmed-meshheading:6091113-Genetic Vectors,
pubmed-meshheading:6091113-Mice,
pubmed-meshheading:6091113-Nucleic Acid Hybridization,
pubmed-meshheading:6091113-Protein Biosynthesis,
pubmed-meshheading:6091113-RNA, Messenger,
pubmed-meshheading:6091113-Thymoma,
pubmed-meshheading:6091113-Thymus Neoplasms,
pubmed-meshheading:6091113-beta-Galactosidase
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pubmed:year |
1984
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pubmed:articleTitle |
Cloning of terminal transferase cDNA by antibody screening.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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